DuoLife Clinical Formula ProBactilardii® is a complex dietary supplement, consisting of 2 complementary capsules. The Synbiotic capsule contains a prebiotic (in the form of acacia fibre) and the proprietary BACTILARDII® probiotic formula, based on 4 bacterial strains and 1 yeast strain, whose viability is guaranteed at the end of the product's shelf life. The Postbiotic capsule - contains a postbiotic in a formula based on butyric acid. DuoLife Clinical Formula ProBactilardii® features not only an innovative composition, but also a patented formula and direct packaging, ensuring maximum protection of microbial strains contained inside the capsule.
DuoLife Clinical Formula ProBactilardii® is a complex dietary
supplement, consisting of 2 complementary capsules. The Synbiotic capsule contains a prebiotic (in the form of
acacia fibre) and the proprietary BACTILARDII® probiotic formula, based on 4 bacterial strains and 1 yeast
strain, whose viability is guaranteed at the end of the product's shelf life. The Postbiotic capsule - contains
a postbiotic in a formula based on butyric acid. DuoLife Clinical Formula
ProBactilardii® features not only an innovative composition, but also a patented formula and
direct packaging, ensuring maximum protection of microbial strains contained inside the capsule.
The intestinal microflora of a healthy human includes approximately 100 trillion (!) beneficial
microorganisms (bacteria, fungi and even viruses), by number exceeding 10 times the total number of cells in the
Up to 500-1000 beneficial species of microorganisms can live in the human
intestines, most in the large intestine. The composition of the microbiota depends on many
factors, however bacteria of the genus Bacteroides, Bifidobacterium and Lactobacillus are almost always dominant.
In addition to genetic determinants, the composition of gut microbiota can be affected mainly by
age, diet, geographical region, sanitation and medicines used.
The composition and abundance of the microbiome can be disrupted by, among other things:
change in lifestyle,
sudden change in diet, e.g. due to
a change in location, travel to tropical or exotic countries with diverse diet.
The health-promoting effects of microorganisms living in the digestive tract are documented by
thousands of clinical studies and scientific reports. They confirm that a well-functioning microbiome benefits:
DuoLife Clinical Formula ProBactilardii® is recommended for:
1. people who want to support the optimal digestive function
on a daily basis, including but not limited to:
people with disorders of the natural
gut microbiota, including constipation or diarrhoea;
people with digestive problems related
to excessive stress;
people with lactose intolerance;
people who want to support natural
people seeking to maintain proper
people who want to support optimal
biosynthesis of B vitamins and vitamin K in the large intestine;
2. people who want to support the functions of their immune
system, including those with reduced immunity due to excessive stress;
3. people who want to support the optimal functions of their central
nervous system, including:
4. people during and after antibiotic treatment (antibacterial
5. people planning trips, especially to exotic and tropical
countries, with a different diet, to be used before and during the trip.
Ingredients found in DuoLife CLINICAL FORMULA PROBACTILARDII® food supplement support:
proper function of the gastrointestinal
tract in healthy people;
proper function of the gastrointestinal
tract during antibiotic therapy;
function of the gastrointestinal tract
during diarrhoea and bacterial, viral and fungal infections;
function of the gastrointestinal tract in
people living under excessive stress;
optimal function of the immune system;
proper function of the gut microbiota;
optimal condition of the large intestine;
proper digestive processes;
optimal biosynthesis of B vitamins and
vitamin K in the large intestine;
function of the central nervous system –
especially optimal mood and emotional balance;
proper lactose metabolism;
proper blood cholesterol level;
removing toxins from the body;
restoration of gut microbiota after
bacterial, viral and fungal infections of the gastrointestinal tract.
What makes DuoLife Clinical Formula ProBactilardii® different?
Proprietary Bactilardii® formula
Stability and viability of microorganisms
Clinical trials on strains
Resistance during passage through the gastrointestinal tract
Synergistic health-promoting effect on the gut-brain axis
Optimal protection of strains
Less is more
Innovative composition – a combination of prebiotic,
probiotic and postbiotic in one product
PROBIOTICS are products containing selected live strains of microorganisms that have a
beneficial effect on the human body by improving the balance of the gut microbiota. The strains contained in
DuoLife Clinical Formula ProBactilardii® have broad, clinically documented health-promoting properties.
PREBIOTICS are products that are selectively used by beneficial microorganisms of the microflora
(mainly intestinal, but not only) in humans, bringing health benefits to the human body. The acacia fibre used
in DuoLife Clinical Formula ProBactilardii® belongs to the soluble fibre fraction. It stimulates the development
of natural intestinal microflora, promotes optimal functions of the digestive tract, supports intestinal
peristalsis, regulating bowel movements and preventing flatulence and chronic constipation.
POSTBIOTICS are defined as the combination of all bioactive ingredients produced by bacteria,
e.g. during fermentation, which are beneficial when introduced into the human body. The butyric acid used in
DuoLife Clinical Formula ProBactilardii® has been shown to have beneficial effects in supporting digestive and
immune function. It is naturally biosynthesized in the large intestine and its supplementation can support the
effects of butyric acid synthesized in the intestines. Butyric acid promotes the optimal condition of the
intestines, supports the intestinal barrier functions, intestinal peristalsis, nourishes intestinal epithelial
cells, can support the body's fight against local inflammation in the intestine.
Proprietary Bactilardii® formula
4 strains of live, freeze-dried, selected and branded bacterial strains (Bifidobacterium
bifidum Rosell® R0071, Bifidobacterium longum Rosell® R0175, Lactobacillus helveticus
Rosell® R0052 and Lacticaseibacillus rhamnosus Rosell® R0011) in DuoLife proprietary proportions plus 1
billion yeast strains (Saccharomyces boulardii CNCM I-1079).
BACTILARDII® is a formula created
exclusively for DuoLife by a world expert in the field of probiotics – the Canadian
Concentrated formula - the total amount of microorganisms is 9 billion CFU = 9x109 CFU (CFU =
colony-forming unit) of LIVE freeze-dried bacteria and yeast cultures GUARANTEED in the formula AT THE
END OF THE PRODUCT'S SHELF LIFE. The concentrated dose provides convenient use, usually 1 time per day both
The concentrated dose provides convenient use, usually 1 time per day both capsules
The combined use involves taking 2 capsules at once – the synbiotic in the
first capsule and the postbiotic in the second capsule.
Laboratory-confirmed viability of bacterial and yeast cultures is guaranteed in the formula at
the end of the product's shelf life.
Stability and viability of micro-organisms
The stability and survivability of the microorganisms has been tested and laboratory confirmed
in a reputable laboratory in the United States.
The performance of individual microbial strains has been documented by NUMEROUS clinical trials,
including at least 30 clinical trials for the specific Lallemand strains used in the product (R0011, R0052,
R0175 and R0071). All clinical studies have also demonstrated a high safety profile of the tested bacterial
strains in humans.
Results of preclinical trials of
strains R0175 and R0052 (7 trials in total), which demonstrated barrier effect of the strains and their
adhesion to intestinal epithelium determining probiotic health-promoting effects
Figure 1: Adhesion of Lacticaseibacillus rhamnosus Rosell® R0011 bacilli (A)
and Lactobacillus helveticus Rosell® R0052 bacilli (B) to the intestinal epithelial cells determining
their probiotic effect. Bacteria are indicated by white arrows. Picture from an electron microscope, based
The results of clinical trials using
the Bifidobacterium bifidum Rosell® R0071 strain
Resistance during passage through the digestive tract
Resistance during passage through the gastrointestinal tract has been confirmed in an
experimental study performed for the R0052 and R0011 strains using an in vitro model (IViDiS, in vitro Digestive
System). It confirms the good stability of the strains in gastric and duodenal environment .
Conclusions: The survival of strains R0052 and R0011 is optimal up to 90 minutes of passage
through the stomach and another 90 minutes through the duodenum, which allows concluding about the stability of
the strains throughout the passage through the upper part of the gastrointestinal tract under physiological
Clinical trials in healthy volunteers taking the dietary supplement with the R0011 strain showed
good survival of bacteria throughout the human gastrointestinal tract .
Conclusions: The number of R0011 strain bacilli in the faeces of the subjects before consuming
the supplement (3 weeks, exclusion) and during consumption (12 days, consumption) and during the one-week
The strains in this product have been used in DuoLife's proprietary proportions and in the full
portions used in many clinical trials.
Synergistic health-promoting effect on the gut-brain
Clinical trials confirm that the combination of the R0175 and R0052 bacterial strains contained
in DuoLife Clinical Formula ProBactilardii® has a positive effect on the gut-brain axis, helping to improve both
gastrointestinal and central nervous system (CNS) function. This combination has a beneficial effect on
psychological functions in healthy people, helping to maintain an optimal mood and promoting protection against
the adverse effects of stress on the function of the gastrointestinal tract and the central nervous system. It
also helps alleviate the intestinal ailments caused by excessive emotions.
Clinical trial results supporting synergistic health-promoting effects on the gut-brain axis,
strain combinations: Bifidobacterium longum Rosell® R0175 and Lactobacillus helveticus Rosell®
R0052. The amounts and proportions of the R0175 and R0052 bacterial strain colonies used in clinical trials
correspond to the amounts contained in the DuoLife Clinical Formula ProBactilardii® preparation.
Figure 2. The synergistic effect of R0052 and R0175 strains has been documented in
clinical trials conducted in 2011 (A) and 2008 (B). Figure A: Improvement of psychological
functions (global mood score; HSCL-90 Global Severity Index) compared to placebo, combined with a decrease in
urinary stress biomarker (cortisol) with 30 days of use for both strains (3x109 CFU in total) in 55 healthy
volunteers. Figure B: Average improvement in gut function in 75 healthy
volunteers exposed to occasional stress during 21 days of using a mixture of R0052 and R0175 strains. Based on
[4-6] Placebo = reference group, not receiving probiotics.
Optimal protection of strains
Proprietary innovative formulation protects strains from adverse conditions and from stomach
acid and bile with proprietary BIO-SUPPORT™ technology. The stability of the strains within the gastrointestinal
tract has been proven in preclinical in vitro tests and in clinical studies. The microgranules are further
protected by delayed-release HPMC capsules, all encapsulated in patented, drug-certified ACTIV-VIALTM
direct packaging with Activ-Polymer™ lining that minimizes the ingress of moisture, light and oxygen to the
capsules in the container, protecting bacterial and yeast strains and other ingredients during product storage
BIO-SUPPORT™ technology action scheme – ensuring protection of all
Patented, innovative, drug-certified ACTIV-VIAL™ direct packaging
No refrigeration required, recommended storage at room temperature 20-25°C.
Including 4 bacterial strains and 1 yeast strain in the form of LIVE freeze-dried
microorganisms. The natural substance that protects the strains against the harmful effects of atmospheric
oxygen is vitamin C of natural origin - L-ascorbic acid (described on the label as an antioxidant, pursuant to
the EU Regulation (EC) 1333/2008).
Less is more
It contains no artificial fillers and is GMO and gluten free.
Suitable for vegans and vegetarians
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What to look for when choosing the best probiotic for you and your loved ones:
Does it contain live bacteria cultures?
What strains does the product contain?
What number of strains does it contain?
Are the strains selected and branded?
Have the strains been clinically tested?
Besides probiotics, does the product
contain prebiotics and a postbiotic?
Does it contain live yeasts and in what
What is the declared live culture content
at the end of the product's shelf life?
Has the product been tested on stability?
Does it contain strains that interact with
the gut-brain-gut axis?
Does the product need to be stored in the
Does the packaging protect against light,
moisture and temperature?
Why is DuoLife Clinical Formula ProBactilardii® the product for you and your loved ones?
Does the product contain prebiotics and a postbiotic in addition to probiotics?
 Sherman, P. M., Johnson-Henry, K. C., Yeung, H. P., Ngo, P. S., Goulet, J., & Tompkins,
T. A. (2005). Probiotics reduce enterohemorrhagic Escherichia coli O157: H7-and enteropathogenic E. coli O127:
H6-induced changes in polarized T84 epithelial cell monolayers by reducing bacterial adhesion and cytoskeletal
rearrangements. Infection and immunity, 73(8), 5183-5188.
 Tompkins, T., Mainville, I., & Arcand, Y. (2011). The impact of meals on a probiotic
during transit through a model of the human upper gastrointestinal tract. Beneficial microbes, 2(4), 295-303.
 Firmesse, O., Mogenet, A., Bresson, J. L., Corthier, G., & Furet, J. P. (2008).
Lactobacillus rhamnosus R11 consumed in a food supplement survived human digestive transit without modifying
microbiota equilibrium as assessed by real-time polymerase chain reaction. Journal of molecular microbiology and
biotechnology, 14(1-3), 90-99.
 Messaoudi, M., Violle, N., Bisson, J. F., Desor, D., Javelot, H., & Rougeot, C. (2011).
Beneficial psychological effects of a probiotic formulation (Lactobacillus helveticus R0052 and Bifidobacterium
longum R0175) in healthy human volunteers. Gut microbes, 2(4), 256-261.
 Messaoudi, M., Lalonde, R., Violle, N., Javelot, H., Desor, D., Nejdi, A., ... &
Cazaubiel, J. M. (2011). Assessment of psychotropic-like properties of a probiotic formulation (Lactobacillus
helveticus R0052 and Bifidobacterium longum R0175) in rats and human subjects. British Journal of Nutrition,
 Diop, L., Guillou, S., & Durand, H. (2008). Probiotic food supplement reduces
stress-induced gastrointestinal symptoms in volunteers: a double-blind, placebo-controlled, randomized trial.
Nutrition Research, 28(1), 1-5.
capsule: proprietary BACTILARDII ® formula, including 9 billion (9x109) CFU* freeze-dried,
selected live bacteria cultures and selected live yeast cultures per capsule, acacia fibre, potato starch –
carrier, vitamin C (L-ascorbic acid) of natural origin 1.5 mg / capsule – antioxidant, coating ingredient:
hydroxypropyl methylcellulose (HPMC). Postbiotic capsule: butyrate granules (as triglyceride) in sunflower oil 380 mg / capsule, expressed as butyric acid 91 mg / capsule, coating ingredients: hydroxypropyl
methylcellulose (HPMC), copper complexes of chlorophylls and chlorophyllins – colouring agent.
Ingredient contents in the daily dose of the product
Synbiotic capsule (prebiotic-probiotic)
BACTILARDII® formula containing:
8 x 109 CFU*
1.6 x 1010 CFU*
Bifidobacterium bifidum Rosell® R0071
Lactobacillus helveticus Rosell® R0052
Lactobacillus rhamnosus Rosell® R0011
Bifidobacterium longum Rosell® R0175
1 x 109 CFU*
2 x 109 CFU*
Saccharomyces boulardii CNCM I-1079
Butyrate (as triglyceride)
expressed as butyric acid
The quantity of live micro-organisms is guaranteed until the expiry date of the
product, provided that the storage conditions indicated on the label are complied with.
Directions for use:Adults and children over 12 years of age: 1 capsule from each container per day to be washed down with cool water. The combined use involves 2 capsules taken simultaneously – the synbiotic in the first capsule and the postbiotic in the second capsule. If necessary, the use of each capsule may be increased to twice a day. Do not exceed the recommended maximum daily dose. Children aged 7 to 12: the product (both capsules) can be used upon prior consultation with a doctor. Children aged 3 to 7 and pregnant or breast-feeding women: using the synbiotic capsules should be consulted with a doctor. The use of postbiotic capsules is not recommended. The product is not intended for children under 3 years of age.
The capsules are only intended for children who can swallow a capsule. In children who cannot swallow the capsule, the contents of the capsule can be poured out and given on a teaspoon, or additionally dissolved in a small amount of water and taken immediately after preparation.
For antibiotic therapy, it is recommended to use the preparation during the period of taking antibiotic, preferably 1 hour after taking the medicine. It is also recommended to continue to take the supplement for 7-10 days following the end of the antibiotic therapy.
For travels abroad, especially to exotic countries and tropical areas, use the preparation 5 days before departure, for the entire stay, and 5 days after returning from abroad.
The product should not be used as a substitute for a varied diet. Balanced diet and healthy lifestyle are the basis for proper functioning of human body.
Storage: Store at room temperature (20-25°C), keep out of the reach of children. It does not require refrigeration.
Precautions: hypersensitivity to any of the product ingredients. Postbiotic capsules should not be used in children under 7 years of age, pregnant or breastfeeding women. Do not use the product in children under 3 years of age.